Studies on reproductive organs which correlate the acid hydrolase and phosphatase enzyme activities and macromolecular concentrations with reproductive status of the female are mostly confined to mammalian systems. No report is available for ovary and oviduct on these parameters in case of a tropical common Indian rock lizard, P. blanfordanus. The present study has been undertaken to quantify the acid and alkaline phosphatases, &-D-galactosidase enzymes, and protein, DNA and RNA contents in the ovary and oviduct, of P. Blanfordanus, to know whether they have any role in reproductive activities, and will be useful in comparative study of vertebrates from the evolutionary point of view. The enzyme activities as well as macromolecular contents are high during breeding period and low during non-breeding period. The results of the present study reveal that these biochemical parameters may have a role in folliculogenesis, vitellogenesis, egg shell formation, oviposition and other reproductive activities.